Article
A mutation in the signal recognition particle 7S RNA of the yeast Yarrowia lipolytica preferentially affects synthesis of the alkaline extracellular protease: in vivo evidence for translational arrest.
The Journal of cell biology - 1 Feb 1992
Yaver D S, Matoba S, Ogrydziak D M
Abstract excerpt
Replacement of the signal recognition particle (SRP) 7S gene (SCR1) on a replicating plasmid with scr1-1 (G to A at 129 and A to T at 131 in the consensus sequence -GNAR- in the loop of domain III) resulted in temperature sensitivity for growth of cells in which both chromosomal SRP 7S RNA genes were deleted. Pulse-chase immunoprecipitation experiments were done after a shift to non-permissive temperature using...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- Protein Precursors
- RNA, Fungal
- Ribonucleoproteins