Article
Structural investigation of Tet repressor loop 154-167: a time-resolved fluorescence study of three single Trp mutants.
Archives of biochemistry and biophysics - 15 Oct 1997
Alberti P, Bombarda E, Kintrup M, Hillen W, Lami H, Piémont E, Doglia S M, Chabbert M
Abstract excerpt
We have studied the time-resolved fluorescence of three engineered Tet repressor (TetR) mutants bearing a single Trp residue at positions 162, 163, and 165 in the C-terminal part of the loop joining helices 8 and 9. Detailed analysis indicates that, at 20 degrees, the fluorescence decay of each Trp can be described as the sum of three exponential components with lifetimes in the 1-, 3-, and 6-ns range. Emission...
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