Article
Recombinant Gq alpha. Mutational activation and coupling to receptors and phospholipase C.
The Journal of biological chemistry - 5 Jan 1992
Conklin B R, Chabre O, Wong Y H, Federman A D, Bourne H R
Abstract excerpt
Gq mediates hormonal stimulation of phosphoinositide-specific phospholipase C (PI-PLC). We mutated the alpha subunit of Gq (alpha q) to replace arginine 183 with cysteine. Mutations that substitute cysteine for the corresponding arginine residues of alpha s and alpha i2 constitutively activate their respective effector pathways, creating the gsp and gip2 oncogenes. Transient expression of alpha q-R183C in COS-7...
Topics
- Adenylyl Cyclase Inhibitors
- Amino Acid Sequence
- Base Sequence
- Cell Line
- Cyclic AMP
- DNA
- Enzyme Activation
- GTP-Binding Proteins
- Gene Expression
- Humans
- Inositol Phosphates