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Reconstitution of complete SV40 DNA replication with purified replication factors.

1 Apr 1994

Abstract excerpt

The identification and purification of human cell proteins required for the production of form I DNA following DNA replication from the simian virus 40 (SV40) origin is described. Using these proteins, complete SV40 DNA replication was reconstituted with only purified DNA replication factors: SV40 large tumor antigen (TAg), replication protein A (RPA), DNA topoisomerases I and II, DNA polymerase alpha-primase,...

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PMID: 8144677
DOI: 10.1016/s0021-9258(17)34146-7

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