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Article

In vivo selection of lethal mutations reveals two functional domains in arginyl-tRNA synthetase.

RNA (New York, N.Y.) - 1 Mar 2000

Geslain R, Martin F, Delagoutte B, Cavarelli J, Gangloff J, Eriani G

Abstract excerpt

Using random mutagenesis and a genetic screening in yeast, we isolated 26 mutations that inactivate Saccharomyces cerevisiae arginyl-tRNA synthetase (ArgRS). The mutations were identified and the kinetic parameters of the corresponding proteins were tested after purification of the expression products in Escherichia coli. The effects were interpreted in the light of the crystal structure of ArgRS. Eighteen...

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Identifiers

PMID: 10744027
DOI: 10.1017/s1355838200992331
PMCID: PMC1369925

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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