Article
Nucleotides that determine Escherichia coli tRNA(Arg) and tRNA(Lys) acceptor identities revealed by analyses of mutant opal and amber suppressor tRNAs.
Proceedings of the National Academy of Sciences of the United States of America - 1 Dec 1990
McClain W H, Foss K, Jenkins R A, Schneider J
Abstract excerpt
We have constructed an opal suppressor system in Escherichia coli to complement an existing amber suppressor system to study the structural basis of tRNA acceptor identity, particularly the role of middle anticodon nucleotide at position 35. The opal suppressor tRNA contains a UCA anticodon and the mRNA of the suppressed protein (which is easily purified and sequenced) contains a UGA nonsense triplet. Opal...
Topics
- Anticodon
- Base Sequence
- Genes, Suppressor
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Transfer
- RNA, Transfer, Arg
- RNA, Transfer, Lys