Article
Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii.
Protein expression and purification - 1 Aug 2003
Chu Xiusheng, Liu Xiaojun, Yau Mabel, Leung Yun-Chung, Li Ding
Abstract excerpt
In microorganisms and plants, mevalonate kinase is involved in the biosynthesis of isoprenoid derivatives, one of the largest groups of natural products. We subcloned the gene of mevalonate kinase from Methanococcus jannaschii into a bacterial expression vector pLM1 with six continuous histidine codons attached to the 5' end of the gene. A variety of mutant expression plasmids including pMMK(R196K), pMMK(R196Q),...
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