Article
Phosphoglycerate mutase from Schizosaccharomyces pombe: development of an expression system and characterisation of three histidine mutants of the enzyme.
Biochimica et biophysica acta - 15 Aug 1996
Nairn J, Price N C, Kelly S M, Rigden D, Fothergill-Gilmore L A, Krell T
Abstract excerpt
The small, monomeric, phosphoglycerate mutase (PGAM) from Schizosaccharomyces pombe has been overexpressed in a strain of Saccharomyces cerevisiae in which the gene encoding PGAM has been deleted, with a yield of purified enzyme of 10-15 mg per litre cell culture. Three mutants in which histidine...
Topics
- Base Sequence
- Circular Dichroism
- Histidine
- Kinetics
- Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Phosphoglycerate Mutase
- Recombinant Proteins
- Schizosaccharomyces
- Spectrometry, Fluorescence