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Article

Identification of essential arginines in the acetate kinase from Methanosarcina thermophila.

Biochemistry - 4 Apr 2000

Singh-Wissmann K, Miles R D, Ingram-Smith C, Ferry J G

Abstract excerpt

Site-directed mutagenesis is a powerful tool for identifying active-site residues essential for catalysis; however, this approach has only recently become available for acetate kinase. The enzyme from Methanosarcina thermophila has been cloned and hyper-produced in a highly active form in Escherichia coli (recombinant wild-type). The role of arginines in this acetate kinase was investigated. Five arginines (R91,...

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Identifiers

PMID: 10736166
DOI: 10.1021/bi991998h

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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