Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression and purification of His-tagged rat mitochondrial medium-chain acyl-CoA dehydrogenase wild-type and Arg256 mutant proteins.

Protein expression and purification - 1 Oct 2004

Zeng Jia, Li Ding

Abstract excerpt

Mitochondrial medium-chain acyl-CoA dehydrogenase is a key enzyme for the beta-oxidation of fatty acids, and the deficiency of this enzyme in patient has been previously reported. We cloned the gene of rat mitochondrial medium-chain acyl-CoA dehydrogenase into a bacterial expression vector pLM1 with six continuous histidine codons attached to the 3' of the gene. The cloned gene was overexpressed in Escherichia...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Acyl-CoA Dehydrogenase
Share to Arginine
Share to Biochemistry

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Circular Dichroism

Share to Fatty Acids

Share to Histidine

Share to Mitochondria

Share to Oxygen

Source

Identifiers

PMID: 15358373
DOI: 10.1016/j.pep.2004.06.021

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression and purification of His-tagged rat mitochondrial medium-chain acyl-CoA dehydrogenase wild-type and Arg256 mutant proteins.PMID 15358373Read ↗

Expression and purification of His-tagged rat mitochondrial short-chain 3-hydroxyacyl-CoA dehydrogenase wild-type and Ser137 mutant proteins.Semantic proximity Read ↗

Molecular characterization of medium-chain acyl-CoA dehydrogenase deficiency causing sudden death.Semantic proximity Read ↗

Mutation of Tyr375 to Lys375 allows medium-chain acyl-CoA dehydrogenase to acquire acyl-CoA oxidase activity.Semantic proximity Read ↗

Identification of the catalytic base in long chain acyl-CoA dehydrogenase.Semantic proximity Read ↗

The molecular basis of medium chain acyl-CoA dehydrogenase deficiency: survey and evolution of 985A----G transition, and identification of five rare types of mutation within the medium chain acyl-CoA dehydrogenase gene.Semantic proximity Read ↗

Clinical and molecular aspects of Japanese children with medium chain acyl-CoA dehydrogenase deficiency.Semantic proximity Read ↗

Characterization of wild-type and an active site mutant of human medium chain acyl-CoA dehydrogenase after expression in Escherichia coli.Semantic proximity Read ↗

Rapid, comprehensive screening of the human medium chain acyl-CoA dehydrogenase geneSemantic proximity Read ↗

Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD). K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein.Semantic proximity Read ↗

Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii.Semantic proximity Read ↗

Molecular analysis of medium chain acyl-CoA dehydrogenase deficiency.Semantic proximity Read ↗

Cloning of glutaryl-CoA dehydrogenase cDNA, and expression of wild type and mutant enzymes in Escherichia coli.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.