Article
A Chinese hamster ovary cell mutant defective in the non-endocytic uptake of fluorescent analogs of phosphatidylserine: isolation using a cytosol acidification protocol.
The Journal of cell biology - 1 Mar 1995
Hanada K, Pagano R E
Abstract excerpt
Transmembrane movement of phosphatidylserine (PS) and various PS analogs at the plasma membrane is thought to occur by an ATP-dependent, protein-mediated process. To isolate mutant CHO cells defective in this activity, we first obtained conditions which inhibited the endocytic, but not the non-en...
Topics
- 4-Chloro-7-nitrobenzofurazan
- Animals
- Biological Transport
- CHO Cells
- Carrier Proteins
- Cell Compartmentation
- Cell Membrane
- Cricetinae
- Cytosol
- Fluorescent Dyes
- Hydrogen-Ion Concentration
- Liposomes
- Microscopy, Fluorescence
- Mutagenesis
- Mutation
- Phosphatidylserines
- Phospholipids
- Selection, Genetic