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Article

Parasexual genetics of Dictyostelium gene disruptions: identification of a ras pathway using diploids.

BMC genetics - 10 Jul 2003

King Jason, Insall Robert H

Abstract excerpt

BACKGROUND: The relative ease of targeted gene disruption in the social amoeba Dictyostelium has stimulated its widespread use as an experimental organism for cell and developmental biology. However, the field has been hamstrung by the lack of techniques to recombine disrupted genes. RESULTS: We describe new techniques for parasexual fusion of strains in liquid medium, selection and maintenance of the resulting...

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Identifiers

PMID: 12854977
DOI: 10.1186/1471-2156-4-12
PMCID: PMC183827

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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