Article
Real-time PCR for chloroquine sensitivity assay and for pfmdr1-pfcrt single nucleotide polymorphisms in Plasmodium falciparum.
Journal of microbiological methods - 1 Sept 2003
de Monbrison Frédérique, Raynaud Delphine, Latour-Fondanaiche Christine, Staal Anne, Favre Sébastien, Kaiser Karine, Peyron François, Picot Stéphane
Abstract excerpt
Plasmodium falciparum drug resistance is a major problem in malaria endemic areas. Molecular markers and in vitro tests have been developed to study and monitor drug resistance. However, none, used alone, can provide sufficient data concerning the level of drug resistance and to issue precise gui...
Topics
- ATP-Binding Cassette Transporters
- Animals
- Antimalarials
- Benzothiazoles
- Chloroquine
- DNA, Protozoan
- Diamines
- Drug Resistance
- Fluorescence Resonance Energy Transfer
- Genetic Variation
- Humans
- Inhibitory Concentration 50
- Malaria, Falciparum
- Organic Chemicals