Article
A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR.
Malaria journal - 7 May 2004
Purfield Anne, Nelson Amy, Laoboonchai Anita, Congpuong Kanungnij, McDaniel Phillip, Miller R Scott, Welch Kathy, Wongsrichanalai Chansuda, Meshnick Steven R
Abstract excerpt
BACKGROUND: Surveillance for drug-resistant Plasmodium falciparum should be a component of malaria control programmes. Real-time PCR methods for the detection of parasite single-nucleotide polymorphisms (SNPs) and gene amplification could be useful survellance tools. METHODS: A real-time PCR assay has been developed that identifies single nucleotide polymorphisms (SNPs) at amino acids 86, 184, 1034 and 1042 in...
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