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Article

[Multiplex PCR for analysis of the Plasmodium falciparum drug resistance molecular markers].

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 1 Dec 2007

Zhang Guo-Qing, Tang Lin-Hua, Guan Ya-Yi, Zhou Shui-Sen, Zheng Bin, Huang Fang, Wu Song, Liu Yan

Abstract excerpt

OBJECTIVE: To develop a multiplex PCR protocol for amplification of five Plasmodium falciparum drug resistance related genes, thereby facilitate the rapid and high throughput analysis of the drug resistance molecular markers. METHODS: Five pairs of primers were designed according to the reference sequences by using Primer Premier 5.0 and Oligo 6.0 software. Drug resistance related genes, including P. falciparum...

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PMID: 18441889

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