Article
Effect of the substitution of critical residues on the allorecognition of HLA-B27.
Clinical and experimental rheumatology - 1 Jan 2000
Ozen S, Clayberger C, Krensky A M, Benjamin R
Abstract excerpt
The three-dimensional structure of the HLA class I molecules has highlighted the importance of the "groove" formed by the helices. We used site-directed mutagenesis to construct a series of HLA-B27 mutants with different substitutions at the sites of the conserved amino acid residues of HLA-B27 s...
Topics
- Amino Acid Sequence
- Cell Line
- Cell Separation
- Epitopes
- Flow Cytometry
- HLA-B27 Antigen
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- T-Lymphocytes, Cytotoxic