Article
Use of a genome-wide approach to identify new genes that control resistance of Saccharomyces cerevisiae to ionizing radiation.
Radiation research - 1 Jul 2003
Game John C, Birrell Geoff W, Brown James A, Shibata Toru, Baccari Clelia, Chu Angela M, Williamson Marsha S, Brown J Martin
Abstract excerpt
We have used the recently completed set of all homozygous diploid deletion mutants in budding yeast, S. cerevisiae, to screen for new mutants conferring sensitivity to ionizing radiation. In each strain a different open reading frame (ORF) has been replaced with a cassette containing unique 20-mer sequences that allow the relative abundance of each strain in a pool to be determined by hybridization to a...
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