Toxicity of 5-aza-2'-deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyltransferase rather than DNA demethylation.

doi:10.1073/pnas.91.25.11797

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Article

Toxicity of 5-aza-2'-deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyltransferase rather than DNA demethylation.

Proceedings of the National Academy of Sciences of the United States of America - 6 Dec 1994

Jüttermann R, Li E, Jaenisch R

Abstract excerpt

The deoxycytidine analog 5-aza-2'-deoxycytidine (5-azadCyd) has been widely used as a DNA methylation inhibitor to experimentally induce gene expression and cellular differentiation. Prior to the availability of mutant mice with altered DNA methyltransferase levels, treatment of cells with drugs has been the only means to experimentally manipulate the level of genomic DNA methylation in mammalian cells....

Topics

Animals
Antineoplastic Agents
Azacitidine
Blotting, Southern
Cell Division
Cell Survival
Cells, Cultured
Crosses, Genetic
DNA
DNA (Cytosine-5-)-Methyltransferases
Decitabine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Embryo, Mammalian

Female

Fibroblasts

Genotype

Heterozygote

Kinetics

Mice

Source

Open in PubMed Open full text

Identifiers

PMID: 7527544
DOI: 10.1073/pnas.91.25.11797
PMCID: PMC45322

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7527544-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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