Article
APRT: a versatile in vivo resident reporter of local mutation and loss of heterozygosity.
Environmental and molecular mutagenesis - 1 Jan 1996
Stambrook P J, Shao C, Stockelman M, Boivin G, Engle S J, Tischfield J A
Abstract excerpt
We describe an in vivo mutagenesis model that utilizes reverse mutation and forward mutation at the endogenous Aprt locus. Reverse mutation provides an in situ method for detecting environments or agents that cause point mutations. Forward mutation detects large chromosomal events, including mitotic recombination, chromosome loss, and large multilocus deletion, all of which can lead to loss of heterozygosity....
Topics
- Adenine
- Adenine Phosphoribosyltransferase
- Adenosine Monophosphate
- Animals
- Autoradiography
- Embryo, Mammalian
- Fibroblasts
- Genes, Reporter
- Heterozygote
- Injections, Intraperitoneal
- Mice