Article
The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco.
Nucleic acids research - 1 Jan 2006
Fojtová Miloslava, Bleys Annick, Bedrichová Jana, Van Houdt Helena, Krízová Katerina, Depicker Anna, Kovarík Ales
Abstract excerpt
We studied the in trans-silencing capacities of a transgene locus that carried the neomycin phosphotransferase II reporter gene linked to the 35S promoter in an inverted repeat (IR). This transgene locus was originally posttranscriptionally silenced but switched to a transcriptionally silenced epiallele after in vitro tissue culture. Here, we show that both epialleles were strongly methylated in the coding region...
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