Article
A novel helper phage that improves phage display selection efficiency by preventing the amplification of phages without recombinant protein.
Nucleic acids research - 1 Jun 2003
Kramer R Arjen, Cox Freek, van der Horst Marieke, van der Oudenrijn Sonja, Res Pieter C M, Bia Judith, Logtenberg Ton, de Kruif John
Abstract excerpt
Phage display is a widely used technology for the isolation of peptides and proteins with specific binding properties from large libraries of these molecules. A drawback of the common phagemid/helper phage systems is the high infective background of phages that do not display the protein of interest, but are propagated due to non-specific binding to selection targets. This and the enhanced growth rates of...
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