Article
A phagemid vector using the E. coli phage shock promoter facilitates phage display of toxic proteins.
Gene - 4 Mar 1999
Beekwilder J, Rakonjac J, Jongsma M, Bosch D
Abstract excerpt
Phage display is a powerful tool with which to adapt the specificity of protease inhibitors. To this end, a library of variants of the potato protease inhibitor PI2 was introduced in a canonical phagemid vector. Although PI2 is a natural trypsin inhibitor, we were unable to select trypsin-binding variants from the library. Instead, only mutants carrying deletions or amber stop codons were found. Bacteria carrying...
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