An expression-packaging-processing vector which selects and maintains 7-kb DNA inserts in the blue T4 phage genome.

Abstract excerpt

We have developed an efficient positive-selection vector to insert foreign DNA segments fused to the T4 ipIII gene (encoding internal protein IPIII) into the bacteriophage T4 genome. By using partial deletions of the T4 e gene, which encodes phage lysozyme, lysozyme activity required for plaque formation is used to select plasmid integrants which restore the e gene. In this work, we demonstrate that DNA inserts...

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