An expression-packaging-processing vector which selects and maintains 7-kb DNA inserts in the blue T4 phage genome.

doi:10.1016/0378-1119(93)90463-d

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Article

An expression-packaging-processing vector which selects and maintains 7-kb DNA inserts in the blue T4 phage genome.

Gene - 22 Dec 1993

Hong Y R, Black L W

Abstract excerpt

We have developed an efficient positive-selection vector to insert foreign DNA segments fused to the T4 ipIII gene (encoding internal protein IPIII) into the bacteriophage T4 genome. By using partial deletions of the T4 e gene, which encodes phage lysozyme, lysozyme activity required for plaque f...

Topics

Bacteriophage T4
Blotting, Southern
Capsid
Capsid Proteins
Genes, Viral
Genetic Vectors
Lac Operon
Muramidase
Phenotype
Recombination, Genetic
Restriction Mapping

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