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Efficient affinity-tagging of M13 phage capsid protein IX for immobilization of protein III-displayed oligopeptide probes on abiotic platforms.

Applied microbiology and biotechnology - 1 Feb 2020

Tong Zhou, Silo-Suh Laura A, Kalalah Anwar, Dawson Paul, Chin Bryan A, Suh Sang-Jin

Abstract excerpt

We developed a genetic approach to efficiently add an affinity tag to every copy of protein IX (pIX) of M13 filamentous bacteriophage in a population. Affinity-tagged phages can be immobilized on a surface in a uniform monolayer in order to position the pIII-displayed peptides or proteins for optimal interaction with ligands. The tagging consists of two major steps. First, gene IX (gIX) of M13 phage is mutated in...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 31900564
DOI: 10.1007/s00253-019-10338-8

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