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Article

Method for one-step preparation of double-stranded DNA template applicable for use with Pyrosequencing technology.

Journal of biochemical and biophysical methods - 31 Jul 2002

Nordström Tommy, Alderborn Anders, Nyrén Pål

Abstract excerpt

A new one-step method for fast and efficient preparation of double-stranded DNA template, suitable for use with Pyrosequencing technology, has been developed. In the new method, two different types of oligonucleotides were used to prevent reannealing of remaining PCR primers to the template: oligonucleotides complementary to the PCR primers and 3'-end modified oligonucleotides with the same sequence as the PCR...

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PMID: 12204412
DOI: 10.1016/s0165-022x(02)00034-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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