Article
The allosteric transition of GroEL induced by metal fluoride-ADP complexes.
Journal of molecular biology - 23 May 2003
Inobe Tomonao, Kikushima Kenji, Makio Tadashi, Arai Munehito, Kuwajima Kunihiro
Abstract excerpt
To understand the mechanism of a functionally important ATP-induced allosteric transition of GroEL, we have studied the effect of a series of metal fluoride-ADP complexes and vanadate-ADP on GroEL by kinetic fluorescence measurement of pyrene-labeled GroEL and by small-angle X-ray scattering measurement of wild-type GroEL. The metal fluorides and vanadate, complexed with ADP, are known to mimic the...
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