Article
Two lines of allosteric communication in the oligomeric chaperonin GroEL are revealed by the single mutation Arg196-->Ala.
Journal of molecular biology - 28 Oct 1994
Yifrach O, Horovitz A
Abstract excerpt
Sequence homology between GroEL and Escherichia coli DNA polymerase I, together with the fact that both proteins bind adenine nucleotides, suggested to us that they may have a similar nucleotide binding site. Arg196 in GroEL corresponds to Arg425 in DNA polymerase I, which is near its nucleotide binding site. Here, we report the striking effects of the mutation Arg196-->Ala in GroEL on its kinetic and allosteric...
Topics
- Adenosine Triphosphate
- Allosteric Regulation
- Allosteric Site
- Amino Acid Sequence
- Arginine
- Base Sequence
- Chaperonin 10
- Chaperonin 60
- Escherichia coli
- Hydrolysis
- Kinetics