Article
Kinetic analysis of ATP-dependent inter-ring communication in GroEL.
Journal of molecular biology - 14 May 2004
Amir Amnon, Horovitz Amnon
Abstract excerpt
Different concentrations of ATP were mixed rapidly with single-ring or double-ring forms of GroEL containing the Phe44-->Trp mutation and the time-resolved changes in fluorescence emission, upon excitation at 295 nm, were followed. Two kinetic phases that were previously found for double-ring GroEL are also observed in the case of the single-ring version: (i) a fast phase with a relatively large amplitude that is...
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