Article
Glu257 in GroEL is a sensor involved in coupling polypeptide substrate binding to stimulation of ATP hydrolysis.
Protein science : a publication of the Protein Society - 1 Jun 2006
Danziger Oded, Shimon Liat, Horovitz Amnon
Abstract excerpt
The ATPase activity of many types of molecular chaperones is stimulated by polypeptide substrate binding via molecular mechanisms that are, for the most part, unknown. Here, we report that such stimulation of the ATPase activity of GroEL is abolished when its conserved apical domain residue Glu257 is replaced by alanine. This mutation is also found to convert the ATPase profile of GroEL, a group I chaperonin,...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Alanine
- Amino Acid Substitution
- Binding Sites
- Chaperonin 60
- Glutamic Acid
- Hydrolysis
- Kinetics
- Lactalbumin
- Mutation
- Peptides
- Protein Structure, Tertiary
- Substrate Specificity