Article
p53 mutations detection in urinary bladder cancer in the Greek population: application of the NIRCA assay.
Journal of experimental & clinical cancer research : CR - 1 Mar 2003
Goudopoulou A, Saetta A, Korkolopoulou P, Patsouris E, Fanourakis G, Miaouli M, Thomas-Tsagli E, Davaris P S
Abstract excerpt
The aim of the present study was to assess the use of an alternative molecular approach for p53 mutation detection and to investigate the usefulness of p53 as a prognostic indicator in bladder cancer. We applied the NIRCA assay, which consists of two-step PCR amplification, transcription of the amplified sequence, hybridisation of the transcripts and treatment with RNAses which recognizes mismatches due to the...
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