Article
Cloning of structural gene of Deinococcus radiodurans UV-endonuclease beta.
Bioscience, biotechnology, and biochemistry - 1 Mar 2003
Kitayama Shigeru, Narumi Issay, Funayama Tomoo, Watanabe Hiroshi
Abstract excerpt
To characterize its enzymic property we cloned and sequenced the gene of Deinococcus radiodurans encoding UV-endonuclease beta, an alternative enzyme to UvrABC repairing damaged DNA. Amino acid substitutions were found in UV-sensitive mutants. The putative amino acid sequence had some similarity with those of eukaryotic UV-endonucleases and with a sequence found in a protein data base of Bacillus subtilis.
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