Article
Mutation of D. radiodurans in a gene homologous to ruvB of E. coli.
Mutation research - 1 Nov 1997
Kitayama S, Kohoroku M, Takagi A, Itoh H
Abstract excerpt
Following the digestion of chromosomal DNA of Deinococcus radiodurans with a restriction enzyme a partial genomic library was constructed using lambda phage as a vector. A phage clone whose DNA can complement the deficiency in a radiation-sensitive mutant of D. radiodurans was isolated. Following the subcloning using phasmid vector, a hybrid plasmid containing 1.2 kb inserted DNA was obtained. After the...
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