A system to determine basepair substitutions at the molecular level, based on restriction enzyme analysis; influence of the muc genes of pKM101 on the specificity of mutation induction in E. coli.

doi:10.1016/0027-5107(85)90205-2

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Article

A system to determine basepair substitutions at the molecular level, based on restriction enzyme analysis; influence of the muc genes of pKM101 on the specificity of mutation induction in E. coli.

Mutation research - 1 Jan 2000

Mattern I E, Olthoff-Smit F P, Jacobs-Meijsing B L, Enger-Valk B E, Pouwels P H, Lohman P H

Abstract excerpt

A system has been developed for the analysis of basepair substitutions that are involved in the reversion of a specific missense mutation. The method is based on the ability of restriction enzymes to recognize and cut specific DNA sequences. Wild-type revertants arising from AT----GC transitions,...

Topics

DNA Restriction Enzymes
Escherichia coli
Genes, Bacterial
Mutation

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