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Article

Determination of hepatitis C virus genotype by Pyrosequencing.

Journal of virological methods - 1 May 2003

Elahi Elahe, Pourmand Nader, Chaung Ramsey, Rofoogaran Ara, Boisver Judie, Samimi-Rad Katayon, Davis Ronald W, Ronaghi Mostafa

Abstract excerpt

A simple sequencing-based assay is described for genotyping of hepatitis C virus (HCV). RT-PCR was employed to amplify a 237-nucleotide-long fragment from the 5' untranslated region (UTR) of the genome using one biotinylated and one normal primer. Subsequent to capture of the PCR products on streptavidin-coated beads, single-stranded DNA separation, and hybridization of sequencing primer, Pyrosequencing was...

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Identifiers

PMID: 12711060
DOI: 10.1016/s0166-0934(03)00068-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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