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Article

A real-time Taqman method for hepatitis C virus genotyping and methods for further subtyping of isolates.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009

Rolfe Kathryn J, Wreghitt Tim G, Alexander Graeme J M, Curran Martin D

Abstract excerpt

The HCV genome is highly heterogeneous; more and more genotypes, each with several distinct subtypes, are being identified around the world. Knowledge of genotype is important for planning of treatment regimes, whereas subtype identification is useful in epidemiological studies and outbreak investigation. We describe HCV genotyping and subtyping assays, based on real-time PCR, that are sensitive, specific, and...

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PMID: 19009253
DOI: 10.1007/978-1-59745-394-3_5

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