Article
Influenza genome analysis using pyrosequencing method: current applications for a moving target.
Expert review of molecular diagnostics - 1 Jul 2009
Deyde Varough M, Gubareva Larisa V
Abstract excerpt
Pyrosequencing is a high-throughput non-gel-based DNA sequencing method that was introduced in the late 1990s. It employs a DNA sequencing-by-synthesis approach based on real-time measurement of pyrophosphate released from incorporation of dNTPs. A cascade of enzymatic reactions proportionally converts the pyrophosphate to a light signal recorded in a form of peaks, known as pyrograms. Routinely, a...
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