Article
Software for machine-independent quantitative interpretation of SSCP in capillary array electrophoresis (QUISCA).
BioTechniques - 1 Dec 2002
Higasa Koichiro, Kukita Yoji, Baba Shingo, Hayashi Kenshi
Abstract excerpt
PCR single-stranded conformational polymorphism (SSCP) analysis is a simple and rapid electrophoretic technique for the sensitive detection of sequence variants of PCR products. Here we describe a cross-platform program package, quantitative interpretation of SSCP in capillary array (QUISCA), which allows semi-automated quantitative detection of sequence variants separated by multicolor fluorescence-based SSCP...
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