Article
NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor.
Proteins - 15 May 2003
Wilkinson Adam, Sayer Heather, Bullard Desmond, Smith Andrew, Day Jonathan, Kieser Tobias, Bowater Richard
Abstract excerpt
Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD(+)-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli. In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD(+) as its cofactor. Expression of either protein...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- DNA Ligases
- Escherichia coli
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Mycobacterium tuberculosis
- NAD
- Oligonucleotides
- Sequence Homology, Amino Acid
- Streptomyces
- Substrate Specificity