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Article

NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor.

Proteins - 15 May 2003

Wilkinson Adam, Sayer Heather, Bullard Desmond, Smith Andrew, Day Jonathan, Kieser Tobias, Bowater Richard

Abstract excerpt

Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD(+)-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli. In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD(+) as its cofactor. Expression of either protein...

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PMID: 12696044
DOI: 10.1002/prot.10361

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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