Article
NAD+-dependent DNA ligase (Rv3014c) from Mycobacterium tuberculosis: novel structure-function relationship and identification of a specific inhibitor.
Proteins - 1 Oct 2007
Srivastava Sandeep Kumar, Dube Divya, Kukshal Vandana, Jha Ashok Kumar, Hajela Kanchan, Ramachandran Ravishankar
Abstract excerpt
Mycobacterium tuberculosis codes for an essential NAD+-dependent DNA ligase (MtuLigA) which is a novel, validated, and attractive drug target. We created mutants of the enzyme by systematically deleting domains from the C-terminal end of the enzyme to probe for their functional roles in the DNA nick joining reaction. Deletion of just the BRCT domain from MtuLigA resulted in total loss of activity in in vitro...
Topics
- Binding Sites
- Cloning, Molecular
- DNA Breaks, Single-Stranded
- DNA Ligases
- Enzyme Inhibitors
- Indoles
- Kinetics
- Mutation
- Mycobacterium tuberculosis
- Structure-Activity Relationship