Article
A phage display-based method for determination of relative affinities of mutants. Application of the actin-binding motifs in thymosin beta 4 and the villin headpiece.
The Journal of biological chemistry - 9 May 2003
Rossenu Stefaan, Leyman Shirley, Dewitte Daisy, Peelaers Danny, Jonckheere Veronique, Van Troys Marleen, Vandekerckhove Joel, Ampe Christophe
Abstract excerpt
We propose phage display combined with enzyme-linked immunosorbent assay as a tool for the systematic analysis of protein-protein interactions by investigating the binding behavior of variants to a partner protein. Via enzyme-linked immunosorbent assay we determine both the amount of fusion protein presented at the phage surface and the amount of complex formed, the ratio of which is proportional to the affinity....
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