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Article

The actin binding site of thymosin beta 4 mapped by mutational analysis.

The EMBO journal - 15 Jan 1996

Van Troys M, Dewitte D, Goethals M, Carlier M F, Vandekerckhove J, Ampe C

Abstract excerpt

We characterized in detail the actin binding site of the small actin-sequestering protein thymosin beta 4 (T beta 4) using chemically synthesized full-length T beta 4 variants. The N-terminal part (residues 1-16) and a hexapeptide motif (residues 17-22) form separate structural entities. In both, we identified charged and hydrophobic residues that participate in the actin interaction using chemical cross-linking,...

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PMID: 8617195
PMCID: PMC449934

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The actin binding site of thymosin beta 4 mapped by mutational analysis.PMID 8617195Read ↗

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