Article
A filamentous phage display system for N-linked glycoproteins.
Protein science : a publication of the Protein Society - 1 Oct 2010
Celik Eda, Fisher Adam C, Guarino Cassandra, Mansell Thomas J, DeLisa Matthew P
Abstract excerpt
We have developed a filamentous phage display system for the detection of asparagine-linked glycoproteins in Escherichia coli that carry a plasmid encoding the protein glycosylation locus (pgl) from Campylobacter jejuni. In our assay, fusion of target glycoproteins to the minor phage coat protein g3p results in the display of glycans on phage. The glyco-epitope displayed on phage is the product of biosynthetic...
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