Article
Comparative interaction kinetics of two recombinant Fabs and of the corresponding antibodies directed to the coat protein of tobacco mosaic virus.
Journal of molecular recognition : JMR - 1 Jan 2000
Chatellier J, Rauffer-Bruyère N, Van Regenmortel M H, Altschuh D, Weiss E
Abstract excerpt
Two recombinant Fab fragments, 57P and 174P, recognizing peptide 134-146 of the coat protein of tobacco mosaic virus have been cloned, sequenced and expressed in Escherichia coli. They differ by 15 amino acid changes in the sequence of their variable region. The interaction kinetics of the Fabs with the wild-type and four mutant peptides have been compared using a BIAcoreTM biosensor instrument. The recombinant...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
