Article
Imaging constitutive exocytosis with total internal reflection fluorescence microscopy.
The Journal of cell biology - 3 Apr 2000
Schmoranzer J, Goulian M, Axelrod D, Simon S M
Abstract excerpt
Total internal reflection fluorescence microscopy has been applied to image the final stage of constitutive exocytosis, which is the fusion of single post-Golgi carriers with the plasma membrane. The use of a membrane protein tagged with green fluorescent protein allowed the kinetics of fusion to be followed with a time resolution of 30 frames/s. Quantitative analysis allowed carriers undergoing fusion to be...
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