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Article

Competition-based cellular peptide binding assays for 13 prevalent HLA class I alleles using fluorescein-labeled synthetic peptides.

Human immunology - 1 Feb 2003

Kessler Jan H, Mommaas Bregje, Mutis Tuna, Huijbers Ivo, Vissers Debby, Benckhuijsen Willemien E, Schreuder Geziena M Th, Offringa Rienk, Goulmy Els, Melief Cornelis J M, van der Burg Sjoerd H, Drijfhout Jan W

Abstract excerpt

We report the development, validation, and application of competition-based peptide binding assays for 13 prevalent human leukocyte antigen (HLA) class I alleles. The assays are based on peptide binding to HLA molecules on living cells carrying the particular allele. Competition for binding between the test peptide of interest and a fluorescein-labeled HLA class I binding peptide is used as read out. The use of...

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PMID: 12559627
DOI: 10.1016/s0198-8859(02)00787-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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