Article
A PIT-1 homeodomain mutant blocks the intranuclear recruitment of the CCAAT/enhancer binding protein alpha required for prolactin gene transcription.
Molecular endocrinology (Baltimore, Md.) - 1 Feb 2003
Enwright John F, Kawecki-Crook Margaret A, Voss Ty C, Schaufele Fred, Day Richard N
Abstract excerpt
The pituitary-specific homeodomain protein Pit-1 cooperates with other transcription factors, including CCAAT/enhancer binding protein alpha (C/EBPalpha), in the regulation of pituitary lactotrope gene transcription. Here, we correlate cooperative activation of prolactin (PRL) gene transcription by Pit-1 and C/EBPalpha with changes in the subnuclear localization of these factors in living pituitary cells....
Topics
- Active Transport, Cell Nucleus
- Animals
- CCAAT-Enhancer-Binding Protein-alpha
- Cell Nucleus
- Cells, Cultured
- Centromere
- DNA-Binding Proteins
- Gene Expression Regulation
- Green Fluorescent Proteins
- Heterochromatin
- Homeodomain Proteins
- Humans