Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Bacterial expression, purification, and in vitro N-myristoylation of fusion hepatitis B virus preS1 with the native-type N-terminus.

Protein expression and purification - 1 Jan 2003

Ma Han-Hui, Yang Li, Yang Xin-Ying, Xu Zheng-Ping, Li Bo-Liang

Abstract excerpt

Very low-level expression of hepatitis B virus (HBV) preS1 with the native-type N-terminus hampered the biochemical and functional studies on its myristoylation. In the present study, the fusion HBV preS1 with the native-type N-terminus and a His6-Tag fused to C-terminus (HBV preS1-HT) was highly expressed in Escherichia coli. This was due to an introduced mutation of the rare codon GGA found in the HBV preS1 to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Acyltransferases
Share to Chromatography, Affinity

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Electrophoresis, Polyacrylamide Gel

Share to Hepatitis B Surface Antigens

Share to Hepatitis B virus

Share to Histidine

Share to Myristic Acid

Share to Protein Precursors

Source

Identifiers

PMID: 12509984
DOI: 10.1016/s1046-5928(02)00541-7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Bacterial expression, purification, and in vitro N-myristoylation of fusion hepatitis B virus preS1 with the native-type N-terminus.PMID 12509984Read ↗

Multistep receptor binding of the hepatitis B virus preS1 domain.Semantic proximity Read ↗

N-terminal myristoylation-dependent masking of neutralizing epitopes in the preS1 attachment site of hepatitis B virus.Semantic proximity Read ↗

Study of the putative fusion regions of the preS domain of hepatitis B virus.Semantic proximity Read ↗

HBV Pre-S1-Derived Myristoylated Peptide (Myr47): Identification of the Inhibitory Activity on the Cellular Uptake of Lipid NanoparticlesSemantic proximity Read ↗

Fine mapping of pre-S sequence requirements for hepatitis B virus large envelope protein-mediated receptor interaction.Semantic proximity Read ↗

Exploring the tolerable region for HiBiT tag insertion in the hepatitis B virus genomeSemantic proximity Read ↗

Structure of the bile acid transporter and HBV receptor NTCP.Semantic proximity Read ↗

Synthesis and assembly of Hepatitis B virus envelope protein-derived particles in Escherichia coli.Semantic proximity Read ↗

Intracellular retention of hepatitis B virus surface protein mutants devoid of amino-terminal pre-S1 sequences.Semantic proximity Read ↗

Efficient inhibition of hepatitis B virus infection by acylated peptides derived from the large viral surface protein.Semantic proximity Read ↗

The Hepatitis B Virus Core Variants that Expose Foreign C-Terminal Insertions on the Outer Surface of Virus-Like Particles.Semantic proximity Read ↗

Protein N-myristoylation in Escherichia coli: reconstitution of a eukaryotic protein modification in bacteria.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.