Article
Bacterial expression, purification, and in vitro N-myristoylation of fusion hepatitis B virus preS1 with the native-type N-terminus.
Protein expression and purification - 1 Jan 2003
Ma Han-Hui, Yang Li, Yang Xin-Ying, Xu Zheng-Ping, Li Bo-Liang
Abstract excerpt
Very low-level expression of hepatitis B virus (HBV) preS1 with the native-type N-terminus hampered the biochemical and functional studies on its myristoylation. In the present study, the fusion HBV preS1 with the native-type N-terminus and a His6-Tag fused to C-terminus (HBV preS1-HT) was highly expressed in Escherichia coli. This was due to an introduced mutation of the rare codon GGA found in the HBV preS1 to...
Topics
- Acyltransferases
- Amino Acid Sequence
- Base Sequence
- Chromatography, Affinity
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Hepatitis B Surface Antigens
- Hepatitis B virus
- Histidine
- Humans
- Molecular Sequence Data
- Mutation
- Myristic Acid