Article
Expression of recombinant proteins in a lipid A mutant of Escherichia coli BL21 with a strongly reduced capacity to induce dendritic cell activation and maturation.
Journal of immunological methods - 15 Jan 2003
Cognet Isabelle, de Coignac Amélie Benoit, Magistrelli Giovanni, Jeannin Pascale, Aubry Jean-Pierre, Maisnier-Patin Karine, Caron Gersende, Chevalier Sylvie, Humbert Frédéric, Nguyen Thien, Beck Alain, Velin Dominique, Delneste Yves, Malissard Martine, Gauchat Jean-François
Abstract excerpt
Mutations in the Escherichia coli (E. coli) and Salmonella lpxM gene have been shown to result in strains which grow normally and which produce a non-myristoylated lipopolysaccharide (nmLPS) with strongly reduced endotoxicity. Using homologous recombination, we inactivated the lpxM gene in BL21 (DE3), a strain widely used for the production of recombinant proteins. This led to a derivative unaffected in its...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.