Article
An attenuating mutation in nsP1 of the Sindbis-group virus S.A.AR86 accelerates nonstructural protein processing and up-regulates viral 26S RNA synthesis.
Journal of virology - 1 Jan 2003
Heise Mark T, White Laura J, Simpson Dennis A, Leonard Christopher, Bernard Kristen A, Meeker Rick B, Johnston Robert E
Abstract excerpt
The Sindbis-group alphavirus S.A.AR86 encodes a threonine at nonstructural protein 1 (nsP1) 538 that is associated with neurovirulence in adult mice. Mutation of the nsP1 538 Thr to the consensus Ile found in nonneurovirulent Sindbis-group alphaviruses attenuates S.A.AR86 for adult mouse neurovirulence, while introduction of Thr at position 538 in a nonneurovirulent Sindbis virus background confers increased...
Topics
- Animals
- Cell Line
- Cricetinae
- Mutation
- Protein Processing, Post-Translational
- RNA, Viral
- Sindbis Virus
- Up-Regulation
- Viral Nonstructural Proteins
- Virulence
- Virus Replication