Requirement for an aromatic amino acid or histidine at the N terminus of Sindbis virus RNA polymerase.

doi:10.1128/JVI.72.3.2310-2315.1998

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Article

Requirement for an aromatic amino acid or histidine at the N terminus of Sindbis virus RNA polymerase.

Journal of virology - 1 Mar 1998

Shirako Y, Strauss J H

Abstract excerpt

The N terminal amino acid of nonstructural protein nsP4, the viral RNA polymerase, is a tyrosine in all sequenced alphaviruses; this is a destabilizing amino acid for the N-end rule pathway and results in rapid degradation of nsP4 produced in infected cells or in reticulocyte lysates. We have con...

Topics

Amino Acids
Animals
Chick Embryo
DNA-Directed RNA Polymerases
Histidine
Mutagenesis
Phenotype
Sindbis Virus
Structure-Activity Relationship
Transfection
Viral Nonstructural Proteins
Viral Plaque Assay

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